The work presents results of three-year research, which was conducted on dark chestnut carbonate easily clay soils of JSC AF «Aktyk» Tselinograd area of Akmola region by studying the reaction of soybean to the conditions of nitrogen nutrition and nitrogen fertilizers. In this work, we identified the main factors which determined efficiency of nitrogen fertilizers, offered the method of determination the optimal level of nitrogen in the soil and its achievements, where the soybean generated the maximum possible harvest in emerging moisture conditions. The method allowed determining the nitrogen deficiency in soil and calculating the required dose of nitrogen fertilizers to achieve an optimal level. Thus, the method allowed to purposefully managing the nitrogen regime of soils considering the biological characteristics of soybean and its requirements to the conditions of nitrogen nutrition. And it was guaranteed by economic efficiency and environmental safety of fertilizers application. The effect of climatic factors on the nitrogen-fixing ability of soybean was revealed.