Twenty-eight mapped SSR markers were used to analyze genetic diversity and population structure of Jordanian barley landraces. The neighbour-joining (NJ) clustering and admixture model-based population structure analysis grouped the Jordanian barley landraces and cultivars according to the inflorescence type. High genetic distance (1GSD=0.779) was observed between the two-row and six-row barley landraces. Polymorphic Information Content (PIC) value of SSRs loci ranged from 0.297 to 0.899, with a mean value of 0.658. The results of this study demonstrate the suitability of SSR data for analysis of genetic diversity and the population structure in barley landraces.