Effect of growing media on macro-propagation of dwarf Cavendish banana was studied in an experiment conducted during 2012 to 2014 at the Farm, Department of Horticulture, Assam Agricultural University, Jorhat, Assam. The experiment was laid out in completely randomized design with three replications under ploy house condition. Evaluation of macro-propagation practice using sawdust as initiation media, supplemented with various biofertilizers resulted in highest number of uniform sized tertiary bud production when the decorticated corm cavity was poured with 40 ppm BAP (4 ml) and the media was supplemented with Bacillus subtilis alone and along with VAM. This treatment also performed better regarding shortest time requirement for production of first primary bud and total number of tertiary suckers, and other vegetative growth characters.