Chlorophyll content in leaves of isabgol (Plantago ovata Forsk.) genotypes viz., GI2, HI-96, PB-80 and HI-5 was studied under salt stress at different EC levels viz., control (without salt), 5 and 10 d/Sm of nutrient supplemented NaCl salt solutions in sand filled polythene bags. The results revealed a significant decrease in chlorophyll ‘a’ and chlorophyll ‘b’ contents with increasing EC levels. Reduction in chlorophyll ‘a’ and chlorophyll ‘b’ contents was maximum in the genotype PB-80 and minimum in the genotype GI-2 indicating the higher tolerance of genotype GI-2 and higher sensitivity of the genotype PB-80 to salt stress among all genotypes studied.