Biological agents play an outstanding and effective role in agricultural systems with the reduction of chemical components’ applications. This study carried out during 2013 to 2015 investigated the efficiency of some bio-growth regulators, with anti-stress activity and plant resistance against diseases, on productivity and safety of four tomato cultivars with the goal of sustainable agriculture. Biological active substances were Epin extra, Zirkon, Tekamin max and Immunotsitofit that were performed as the following levels: soaking in 0.2% solution for 6 h, soaking in a 0.2% solution for 6 h and one time foliar treatment, soaking in a 0.2% solution for 6 h and two times foliar treatments, one time foliar treatment, two times foliar treatments and control as a water treatment, respectively. Tomato (Lycopersicon esculentum Mill.) cultivars in this study were Sultan F1, Lodzheyn, Dar Zavolzhja and Beta. Results of this study revealed that Epin extra caused the highest germination of Sultan F1 and Dar Zavoljia significantly; germination of Beta was improved when Tekamin max [(TM3) soaking in a 0.2% solution for 6 h and two times foliar treatment] was used. Bio-agent Tekamin max in all cases had favourable improvement on tomato morphology of all tomato cultivars. Three records of observation on blight spread on tomato cultivar Dar Zavoljia concluded that blight disease was significantly diminished when Tekamin max [(TM3) soaking in a 0.2% solution for 6 h and two times foliar treatment] was performed. Overall, Tekain max as a biological growth regulator was distinguished as the most efficient on growth and development of tomato, and also the lowest blight infection was observed when Tekamin max was applied.