A field experiment was carried out at Model Floriculture Centre, G. B. P. U. A. & T., Pantnagar, Uttarakhand, India during the year 2014 to study the response of growing substrates and growth regulators on stem cuttings of damask rose (Rosa damascena Mill.) cv. ‘Ranisahiba’ with 35 treatment combinations comprising growing substrates viz., soil, soil+FYM, soil+vermicompost, soil+cocopeat, soil+rice husk and plant growth regulators viz., IBA 500 ppm, IBA 1000 ppm, IBA 1500 ppm, NAA 500 ppm, NAA 1000 ppm, NAA 1500 ppm and control. Findings of the present investigation revealed that among growing substrates soil+vermicompost planted cuttings gave best results in most of the parameters like days to sprouting (19.76), maximum sprouting percentage (79.08), survival percentage (87.28), diameter of sprout (3.31 mm), length of sprouts (66.21 cm), number of leaves (23.39), fresh weight (22.41 g) and dry weight of shoot (10.37 g), Among different concentrations of plant growth regulators, best results were obtained with application of IBA 1000 ppm, whereas IBA 500 ppm gave best results with parameters viz., survival percentage (78.83) and days taken to sprouting (19.63).