An experiment was conducted to characterize the physico-chemical quantitative traits of different varieties and new accessions of mango for characterization during the years 2014 and 2016 at GBPUA & T, Pantnagar under tarai region of Uttarakhand. The 40 mango varieties including new accessions were selected for the experimentation. The trial was conducted in a randomized block design with three replications. The results indicated that significantly wide variations were observed among the mango varieties with respect to different physico-chemical quantitative traits of fruits. The fruit weight, length, width, pulp weight and pulp: stone ratio varied from 20.07 to 420.65 g, 3.48 to 13.28 cm, 2.81 to 8.70 cm, 13.15 to 351.64 g and 2.50 to 10.33, respectively. The higher fruit weight (420.65 g), pulp weight (351.64 g) and pulp: stone ratio (10.33) were observed in Mallika, while, maximum length of fruit was recorded in Bangalora (13.28 cm) and width (8.70 cm) in Neelgoa. However, stone weight, length and width ranged from 5.34 to 64.90 g, 2.87 to 10.64 cm and 1.92 to 5.48 cm, respectively. However, the minimum stone weight (5.34 g), stone length (2.87 cm) and width (1.92 cm) were found in Angoor Lata. The TSS, total sugar, non-reducing sugar and total carotenoids varied from 12.06 to 22.32°B, 11.36 to 20.69%, 8.32 to 14.62% and 1.78 to 9.23 mg/100 g, respectively. The higher TSS (22.32°B), total sugar (20.69%), non-reducing sugar (14.62%) and total carotenoid (9.23 mg/100 g) were recorded in Amrapali. The lower acidity (0.05%) was observed in Himsagar. Therefore, it can be concluded that the Mallika and Amrapali varieties of mango have excelled in terms of physical & chemical quantitative traits, respectively. Thus, these can be used for commercial cultivation as well as for further use in breeding programme for quality improvement of mango.