The research work was carried out at University Research Farm, Tamil Nadu Veterinary & Animal Sciences University, Madhavaram during 2014 to evaluate the nitrogenous fertilization on leaf anatomical parameters of bajra napier hybrid grass. The experiment was carried out in sandy soil with treatments T1: Organic alone, T2: 100% recommended dose of fertilizer along with organic nutrients, T3: 50% of recommended dose of fertilizers along with organic, T4: 100% of recommended dose of fertilizer alone and T5: Control. The design was completely randomized with four replications. Leaf parameters such as leaf width, length, leaf area and specific leaf area were computed. The anatomical parameters calculated were epidermis, vascular tissues, mesophyll, schlerenchyma and parenchymatous bundle sheath cells. The results showed that application of 100% recommended dose of fertilizer along with organic nutrients was found to have higher leaf parameters and anatomical structures which indicate the necessity of nitrogen application for better productivity of bajra napier hybrid grass.