The present investigation deals with the morphological and physico-chemical characterization of eight guava genotypes. Significant variations were present with reference to various morphological and biochemical characteristics among the genotypes. Different plant characters ranged as; tree height from 2.04 m in RCGH-11 to 3.52 m in RCGH-1, tree spread from 2.88 m in RCGH-11 to 6.13 m in RCGH-1, stem girth from 22.67 cm in MPUAT-Sel-2 to 33.65 cm in RCGH-1, cross sectional trunk area from 34.49 cm2 in MPUAT Sel-2 to 71.25 cm2 in RCGH-1, number of petals per flower from 4.2 in Arka Kiran to 10.20 in RCGH-1, number of stamens per flower from 440.4 in MPUAT Sel-2 to 536.60 in RCGH7, leaf length from 10.75 cm in RCGH-11 to 13.96 cm in MPUAT Sel-1, leaf width from 4.36 cm in CISH-G-25 to 7.08 cm in MPUAT Sel-2, length: width from 1.87 in RCGH-11 to 2.73 in RCGH-1, leaf area from 65.1 cm2 in CISH-G-35 to 95.71 cm2 in MPUAT Sel-2, number of veins from 19.60 in MPUAT Sel-2 to 26.60 in RCGH-11, leaf fresh weight from 1.10 g in MPUAT Sel-1 to 1.99 g in MPUAT Sel-2, leaf dry weight from 0.66 g in MPUAT Sel-1 to 1.02 g in MPUAT Sel-2, chlorophyll from 0.005 mg/g in Arka Kiran to 0.010 mg/g in CISH-G-35, nitrogen from 0.134% in Allahabad Safeda to 0.329% in MPUAT Sel-2, phosphorus from 0.119% in RCGH-7 to 0.179% in MPUAT Sel-2, potassium from 0.202% in RCGH-11 to 0.812% in CISH-G-35, boron from 49.90 ppm in Arka Kiran to 76.67 ppm in RCGH-7, zinc from 140.24 ppm in Allahabad Safeda to 279.55 ppm in Arka Kiran, iron from 645.67 ppm in Allahabad Safeda to 1382.23 ppm in Arka Kiran, leaf colour from light green in MPUAT Sel-1 and RCGH-1 and dark green in CISH-G-35 and RCGH-11 to green in rest. However, petiole length did not vary significantly. Leaf shape varied from lanceolate in MPUAT Sel-1, oblanceolate in Arka Kiran and RCGH-1 to oblong in rest, leaf apex from acute in MPUAT Sel-1, rounded in CISH-G-35 to obtuse in rest, leaf base from obtuse in MPUAT Sel-1 to rounded in rest, colour of young leaves from light green in Allahabad Safeda, dark green in RCGH-11 to green in rest, tree growth habit from spreading in MPUAT Sel-1, MPUAT Sel-2 and Arka Kiran to upright in rest and flower colour remained white among different genotypes.