A field experiment was conducted growing Indian mustard with added doses of sulphur in the form of bentonite-S and single super phosphate (SSP) to evaluate effect of S addition on different forms of S in an acid soil under mustard cultivation during 2013–14. The preponderance of S in initial soil followed the order: total sulphur> organic sulphur> non-sulphatesulphur> heat soluble sulphur> adsorbed sulphur> water soluble sulphur>sulphate sulphur. The analysis of soil after harvest showed that all S forms decreased in the control plot. The total S content was increased with addition of S but organic S content decreased in all treatments. Although, this decrement was lower down with addition of S. The status of the SO4−2-S in the soil at harvest had no much variation across the treatments. The bentonite S showed the better S use efficiency by the plant.