In this research, the effect of bacterial inoculation was investigated on growth traits, yield and gel qualitative traits of Aloe vera. Experiment was carried out under greenhouse conditions in 2011–12 using a completely randomized block design including eight treatments and three replications. The experimental treatments included plants inoculation with Azotobacter chroococcum strain 12 (A), Pseudomonas florescens strain 41 (P), Azospirillum brasilense (AZ), the dual combination of bacteria A+P, A+AZ and P+AZ, and also a combination of all three bacteria studied (A+P+AZ), and finally the control, which did not use any bio-fertilizer. The findings suggested that use of bio-fertilizers (A, P, A+P+AZ) led a significant increase in gel weight of leaves, plant weight, root length, aloenin and barbaloin of gel in comparison with the control. Of course, they had any significant effect on leaves number in comparison to control. Generally, the research showed that use of bio-fertilizers A, P, A+P+AZ could have a beneficial and effective role in promoting the qualitative and quantitative traits of Aloe vera.