Abstract

MYB1 transcription factor plays significant role in regulating plant secondary metabolisms. microRNA is a class of endogenous small non-coding RNA and negatively regulates gene expression by inhibiting translation of the target mRNA. In this study, MYB1 from four plant species including Morella rubra, Orobanche ramosa, Leucaena leucocephala and Epimedium sagittatum, was investigated via bioinformatic tools and methods. We analyzed their chemical formulas, physico-chemical properties, subcellular localization, transit peptides, transmembrane domains, hydrophobicity, molecular phylogenetic tree, protein structures and predicted the upstream target regulatory miRNA. The results showed that MYB1 genes contained a 5′, 3′ untranslated region and an ORF, respectively. MYB1 proteins were hydrophobic, localized in cytoplasmic matrix with no transit peptides, and their secondary structures were mainly comprised of random coil and α-helix. 3-D models of the four MYB 1 were constructed and four upstream target regulatory miRNAs, miR159, miR5658, miR5021 and miR828, were predicted using the latest miRBase database.