Pre-harvest sprouting (PHS) of Korean wheat cultivars was evaluated for five years and allelic variations of functional markers associated with seed dormancy and PHS, TaVp-1A, TaSdr-B1 and TaPHS1, were also evaluated in this study to improve PHS resistance in Korean wheat breeding programmes. PHS resistance was evaluated using mist spray in humid chamber (PHS-MS) over five years. PHS-MS were significantly influenced by year, genotype and the interaction between year and genotype. PHS-MS ranged from 1.57 to 84.38%. Red-grained wheat cultivars showed lower PHS-MS (11.39%) than white-grained cultivars (62.24%). There was no significant difference in PHS value of Korean wheat cultivars carried different TaVp-1A alleles although five alleles were found in Korean wheat cultivars. The frequency of TaSdr-B1a and TaSdr-B1b allele in Korean wheat cultivars was 79.2 and 20.8%, respectively. TaSdr-B1a allele showed lower PHS value (16.50%) than TaSdr-B1b allele (52.99%). For TaPHS1 allele, there were two types of haplotypes I and II in Korean wheat cultivars. There was non-significant difference of PHS resistance in Korean wheat cultivars carried between haplotype I (27.19%) and haplotype II (20.45%).