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Development and applications of a set of chromosome-specific DNA markers in sesame

DOI: 10.5958/2348-7542.2014.00141.7    | Article Id: 029 | Page : 481-486
Citation :- Development and applications of a set of chromosome-specific DNA markers in sesame. Res. Crop. 15: 481-486
Yanyang Liu, Chengqi Cui, Hongxian Mei, Yongzhan Zheng sesame168@163.com
Address : Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou-450 002, China

Abstract

To identify all sesame chromosomes, a multicolour fluorescence in situ hybridization procedure was developed to delineate and physically map the probes of simple sequence repeat (AC)8, 45S rDNA, 5S rDNA, cot-1 DNA and telomeric repeat (TTTAGGG)n. The results showed that the karyotype formula of this species was found to be 2n=6m+18sm+2st. All 13 chromosomes were identified by the banding and colour pattern in the three examined varieties. The FISH analysis of rDNA, (AC)8 and Cot-1 DNA sites showed two sites for 5S rDNA, three sites for 45S rDNA, nine sites for (AC)8 and ten sites for cot-1 DNA. The (AC)8 on the long arm, pericentromeric region of short arm, at the centromeric and near telomeres at the short arm was assigned number 1, 2, 8 and 12, respectively, by simultaneous hybrization with a Cot-1 DNA site. On chromosome 3, we found two (AC)8 sites. One chromosome with no other hybridization signals but a (AC)8 site on the short arm near telomeres was assigned number 11. Both chromosome numbers 5 and 7 had the 45S rDNA and Cot-1 DNA sites near telomeres at the short arm, while the chromosome number 5 was longer than the 7th. The chromosome numbers 6 and 9 had the (AC)8, 5S rDNA and Cot-1 DNA sites near telomeres at the short arm, while the chromosome number 6 was longer than the 9th. The chromosome with the 45S rDNA site at the telomeres and Cot-1 DNA site near the telomeres at the short arm was identified as number 10. The chromosomes with no other hybridization signals but telomeres sites were identified 4 and 13, while the chromosome number 4 was longer than the 13th. It is hoped that the results in this study would provide the reference and support for further studies in genetics and evolution in sesame.

Keywords

FISH  karyotype  mitotic metaphase chromosomes  Sesamum indicum L.

References

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