GC-MS-MS profiling and toxicity of ethyl acetate extracts of some botanicals against rice weevil, Sitophilus oryzae (L.) in stored maize 

DOI: 10.31830/2454-1761.2023.CR-913    | Article Id: CR-913 | Page : 194-201
Citation :- GC-MS-MS profiling and toxicity of ethyl acetate extracts of some botanicals against rice weevil, Sitophilus oryzae (L.) in stored maize. Crop Res. 58: 194-201
Address : Department of Agricultural Entomology, School of Agriculture, VELS Institute of Science, Technology & Advanced Studies (VISTAS), Velan Nagar, Chennai-600 117 (Tamil Nadu), India
Submitted Date : 19-05-2023
Accepted Date : 28-06-2023


Ethyl acetate extracts of certain botanicals were evaluated in the laboratory for their contact and oral toxicity at 5% concentration against Sitophilus oryzae L. at Natural Pesticide Laboratory, Department of Agricultural Entomology, Agricultural College and Research Institute, Madurai, Tamil Nadu during 2019-21. The result revealed that all the botanicals were effective against S. oryzae compared to the control. Considering the contact toxicity, 5% ethyl acetate extract of Mentha spicata (86.67%) performed better 72 h after treatment. Vitex negundo and Ocimum sanctum caused (83.33%) mortality 72 h after treatment concerning oral toxicity. Mentha spicata caused 93.33% mortality after 15 days of treatment, whereas Vitex negundo, Ocimum sanctum and Tagetes erecta leaf caused 90.00% mortality, which was statistically at par with Mentha spicata. GC-MS-MS study revealed the presence of carvone (38.27%) at the retention time of 7.644, Hexadecanoic acid (2.13%) at the retention time of 27.600, Isopulegol (1.41%) at the retention time of 27.290 and phytochemicals with insecticidal potential in Mentha spicata.Whereas in Ocimum sanctum, Eugenol (59.72%) was the major compound at the retention time of 9.9. Hence, it was concluded that among the tested ethyl acetate extracts, Mentha spicata at 5% was the most effective as a contact and oral toxicant against S. oryzae in stored maize, which possesses the bioactive principle of carvone and it can be developed as an eco-friendly botanical formulation.


Botanicals carvone ethyl acetate Mentha spicata Sitophilus oryzae toxicity


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